109 Awardee Talk: Dietary Supplementation of <i>Bacillus Amyloliquefaciens</i> Influenced Systemic Immunity and Intestinal Microbiome of Weaned Pigs

Abstract Supplementation of various Bacillus spp. has shown to improve growth performance, immune responses, and gut health newly weaned pigs. The present study investigated the effects dietary supplementation 109 CFU/kg (B.) amyloliquefaciens on diarrhea, systemic immunity, intestinal microbiome pigs with or without enterotoxigenic Escherichia coli (ETEC) challenge. An antibiotic treatment was also included in experiment compare efficacy B. vs. carbadox (50 mg/kg). ETEC challenge reduced (P &amp;lt; 0.05) rate but increased frequency diarrhea throughout experiment. tended 0.10) increase performance had limited impacts white blood cell (WBC) count d 7 21 post-inoculation (PI), while reduce WBC PI PI, compared control. No differences were observed immunity when comparing carbadox. antibiotics different microbiome. relative abundance Lactobacillus feces Prevotella PI. Prior challenge, Lachnospiraceae less abundant supplemented than group 0 before infection. Enterobacteriaceae fecal samples lowest among all 0. On 14 Actinobacteria Ileal digesta collected analyze microbiota diversity changes treatments. Shannon index greater ileal Firmicutes Proteobacteria digesta. Under lower Clostridiaceae ileum In conclusion, similar comparison